Play as Cow Girl Anne:
Successfully complete survival mode two or more times.
Play as Way of the Samurai 1 and 2:
Successfully complete all levels (including the extra levels) under the super hard difficulty setting.
Other bonus characters:
Find the wanted posters in the following levels to unlock the corresponding character.
Hard level 8 (Saloon): Found over the chandelier
Hard level 11 (Cactus Gulch): Get to a position where the train station is behind you. Look on the terrace of the building to the left and diagonal from your location.
Hard extra level 2 (Coal Mine): Above the coal mine entrance.
Hard level 9 (Coal mine): From the starting location, search the building directly in front, and beyond the slippery ground.
Hard level 12 (Wilderness): Found in the air next to the bridge.
Hard level 15 (Fort): Search the back of the right side of the fort.
Hard level 16 (Goldberg Residence): Look behind the back stairs inside Goldberg's room.
Easy extra level 1 (Fort): Found diagonally above the statue.
Hard extra level 4 (Coal Mine): Look behind the bridge.
Hard level 7 (Cactus Gulch): Search inside the small room along the tracks.
Hard level 14 (Wilderness): Look around the entrance to the level.
Hard level 2 (Saloon): Search down by the stairs.
Hard level 1 (Cactus Gulch): Found above the general store next to the saloon.
Hard level 3 (Cactus Gulch): Hidden in the back of the small path next to the train station,
Hard level 6 (Fort): Found in an opening by the entrance to the courtyard.
Hard level 4 (Ghost Town): From your starting position, look behind the road blocking sign in front of you.
Hard level 5 (Goldberg Residence): Found behind a door.
Faceless (type 2)
Hard level 13 (Saloon): Look on the pipe next to the stage.
Hard extra level 4 (Coal Mine): Go past the bridge and enter the room with a crane. It is on the wall to the right.
Hard level 10 (Ghost Town): Found in mid-air in the cemetery.
Hard level 9 (Coal Mine): Go past the bridge and look above the dead end to the right.
Hard level 16 (Goldberg Residence): On the first floor, search the pipe on the back left.
Normal extra level 5 (Wilderness): Look at the top of the hill.
Normal level 16 (Wilderness): Look in the dining room, behind the screen.
Hard level 10 (Ghost Town): Search inside the bathroom.